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Culture Plate Gang Continued

Conflicts of Interest: PLANKTONIC (PP) vs. BIOFILM (PBF)
Microbial Natural Selection

Planktonic Phyllis
Biofilm Bradford
Planktonic (PP) Biofilm (PBF)
1.
Individual Level (Simpson's Paradox)
Kin Selection
VS.
Group Level Selection
Group Selection
2.
Competitive Behavior
VS.
Cooperation
3.
Resources consumption
Fitness of Individual
VS.
Economical Use
Fitness of Groups
4.
No Diversity
VS.
Population heterogeneity
5.
Quorum Sensing
VS.
Diffusion Sensing
6.
Antibiotic Resistance via genetics
VS.
Collective Action (Phenotypic)
7.
Growth Restraint (Contact Inhibition)
VS.
Channel Formation
8.
Decreased Virulence
VS.
Increased Virulence as group beneficial trait
Biofilm Bradford

Bioflim Bradford

  • DEPENDENT
  • EARLIEST, organized community (Survival)
  • Legacy of Microbiology, its parent
  • Anti-Koch – “WE” – Kropotkin
  • Devices, Surfaces, Indwelling
  • Chronic Infections
  • Antibiotic Resistance
  • A or B
  • Pathogenecity: Stage of Community (I-IV)
Planktonic Phyllis

Planktonic Phyllis

  • INDEPENDENT
  • Evolved later, Gradually, expensive
  • Transmission efficient
  • Koch Postulate, Pasteur, Darwin “I”
  • Fluids, aerosols
  • Acute Infections
  • Antibiotic Responsive
  • A or B
  • Pathogenicity: Organism structure, Virulence factors
Phyllis and Bradford

Similarities

Linking Biofilm & Planktonic Phenotypes:

  • VAP ↔ Chronic Wounds
  • Biphasic
  • Early/Late
  • Gram + → Gram –
  • Yeast bridge
  • Critical Colonization (Ratio)
  • Cyclical
  • Stage IV

Take Home Message

  1. Read International Literature
  2. Use my Website!
  3. Newer Methods: Redefine microbial Normal Flora
  4. Benefit of Normal Flora (Pre-/Probiotics)
  5. Decreased CHX – Be careful what you wish for!
  6. VAP – Dental Oral Care